Anti-NR2F6 Antibody-Primary Antibody-Cohesion Biosciences

Western blot analysis of NR2F6 expression in HEK293T (A) whole cell lysates.
Immunohistochemical analysis of NR2F6 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of NR2F6 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Product Name	Anti-NR2F6 Antibody
Cat No:	CPA1395
Source:	Rabbit
Reactivity:	H, M, R, B, D, P
Applications:	WB, IH, IF/IC
*Application Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
*Species Reactivity Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish

Size

Price

200 μl

$350

100 μl

$220

30 μl

$110

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Description: Rabbit polyclonal antibody to NR2F6

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human NR2F6. The exact sequence is proprietary.

Purification: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100), IF/IC (1/50 - 1/200)

Gene Symbol: NR2F6

Alternative Names: EAR2; ERBAL2; Nuclear receptor subfamily 2 group F member 6; V-erbA-related protein 2; EAR-2

Entrez Gene (Human): 2063

Entrez Gene (Mouse) : 13864

Entrez Gene (Rat) : 245980

SwissProt (Human): P10588

SwissProt (Mouse) : P43136

SwissProt (Rat) : O09017

Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Western blot analysis of NR2F6 expression in HEK293T (A) whole cell lysates.
Immunohistochemical analysis of NR2F6 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of NR2F6 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Regulation of renin expression by the orphan nuclear receptors Nr2f2 and Nr2f6

Journal AM J PHYSIOL-RENAL

IF 4.2

Application WB

Reactivity

PMID 22278040

The intestinal nuclear receptor signature with epithelial localization patterns and expression modulation in tumors

Journal Gastroenterology

IF 29.4

Application IHC-P

Reactivity Human

PMID 19818784

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