Description: Rabbit polyclonal antibody to ELF5 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human ELF5. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500), IP (1/10 - 1/100) Gene Symbol: ELF5 Alternative Names: ESE2; ETS-related transcription factor Elf-5; E74-like factor 5; Epithelium-restricted ESE-1-related Ets factor; Epithelium-specific Ets transcription factor 2; ESE-2Entrez Gene (Human): 2001Entrez Gene (Mouse) : 13711SwissProt (Human): Q9UKW6SwissProt (Mouse) : Q8VDK3Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of ELF5 expression in HeLa (A), HuvEc (B), Jurkat (C) whole cell lysates.
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  Immunofluorescent analysis of ELF5 staining in HuvEc cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.