Description: Rabbit polyclonal antibody to EIF4E Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human EIF4E. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: EIF4E Alternative Names: EIF4EL1; EIF4F; Eukaryotic translation initiation factor 4E; eIF-4E; eIF4E; eIF-4F 25 kDa subunit; mRNA cap-binding proteinEntrez Gene (Human): 1977Entrez Gene (Mouse) : 13684Entrez Gene (Rat) : 117045SwissProt (Human): P06730SwissProt (Mouse) : P63073SwissProt (Rat) : P63074Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of EIF4E expression in HEK293T (A), Hela (B), mouse lung (C), mouse kidney (D), rat lung (E), rat kidney (F) whole cell lysates.
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  Immunohistochemical analysis of EIF4E staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.