Description: Rabbit polyclonal antibody to EHHADH Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human EHHADH. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: EHHADH Alternative Names: ECHD; Peroxisomal bifunctional enzyme; PBE; PBFEEntrez Gene (Human): 1962Entrez Gene (Mouse) : 74147Entrez Gene (Rat) : 171142SwissProt (Human): Q08426SwissProt (Mouse) : Q9DBM2SwissProt (Rat) : P07896Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of EHHADH expression in HEK293T (A), K562 (B), rat liver (C), rat kidney (D) whole cell lysates.
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  Immunohistochemical analysis of EHHADH staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.