Description: Rabbit polyclonal antibody to EGR Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human EGR1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500) Gene Symbol: EGR1; EGR2 Alternative Names: EGR1; KROX24; ZNF225; Early growth response protein 1; EGR-1; AT225; Nerve growth factor-induced protein A; NGFI-A; Transcription factor ETR103; Transcription factor Zif268; Zinc finger protein 225; Zinc finger protein Krox-24; EGR2; KROX20; E3 SUMO-protein ligase EGR2; AT591; Early growth response protein 2; EGR-2; Zinc finger protein Krox-20Entrez Gene (Human): 1958; 1959Entrez Gene (Mouse) : 13653; 13654Entrez Gene (Rat) : 24330; 114090SwissProt (Human): P18146; P11161SwissProt (Mouse) : P08046; P08152SwissProt (Rat) : P08154; P51774Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of EGR expression in Hela (A), PC3 (B), rat ovary (C), MCF7 (D) whole cell lysates.
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  Immunohistochemical analysis of EGR staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Immunofluorescent analysis of EGR staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.