Description: Rabbit polyclonal antibody to Ephrin B1 (Phospho-Y317) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y317 of human Ephrin B1 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: EFNB1 Alternative Names: EFL3; EPLG2; LERK2; Ephrin-B1; EFL-3; ELK ligand; ELK-L; EPH-related receptor tyrosine kinase ligand 2; LERK-2Entrez Gene (Human): 1947Entrez Gene (Mouse) : 13641SwissProt (Human): P98172SwissProt (Mouse) : P52795SwissProt (Rat) : P52796Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Ephrin B1 (Phospho-Y317) expression in zebrafish (A) whole cell lysates.
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  Immunohistochemical analysis of Ephrin B1 (Phospho-Y317) staining in human colorectal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.