Description: Rabbit polyclonal antibody to Dishevelled 3 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Dishevelled 3. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: DVL3 Alternative Names: KIAA0208; Segment polarity protein dishevelled homolog DVL-3; Dishevelled-3; DSH homolog 3Entrez Gene (Human): 1857Entrez Gene (Mouse) : 13544SwissProt (Human): Q92997SwissProt (Mouse) : Q61062Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Dishevelled 3 expression in PC12 (A), CT26 (B), HCT116 (C), SGC7901 (D) whole cell lysates.
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Immunohistochemical analysis of Dishevelled 3 staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.