Description: Rabbit polyclonal antibody to Dopamine Receptor D1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Dopamine Receptor D1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100), IF/IC (1/50 - 1/200) Gene Symbol: DRD1 Alternative Names: D(1A) dopamine receptor; Dopamine D1 receptorEntrez Gene (Human): 1812Entrez Gene (Mouse) : 13488Entrez Gene (Rat) : 24316SwissProt (Human): P21728SwissProt (Mouse) : Q61616SwissProt (Rat) : P18901Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Dopamine Receptor D1 expression in A549 (A), U2OS (B), mouse kidney (C), rat liver (D) whole cell lysates.
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  Immunohistochemical analysis of Dopamine Receptor D1 staining in human colorectal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Immunofluorescent analysis of Dopamine Receptor D1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.