Description: Rabbit polyclonal antibody to DLX5 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human DLX5. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100), IF/IC (1/50 - 1/200) Gene Symbol: DLX5 Alternative Names: Homeobox protein DLX-5Entrez Gene (Human): 1749Entrez Gene (Mouse) : 13395Entrez Gene (Rat) : 25431SwissProt (Human): P56178SwissProt (Mouse) : P70396SwissProt (Rat) : P50575Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of DLX5 expression in Hela (A), A2780 (B), mouse lung (C), mouse brain (D), mouse kidney (E), rat kidney (F) whole cell lysates.
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Immunohistochemical analysis of DLX5 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Immunofluorescent analysis of DLX5 staining in Raw264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.