Description: Rabbit polyclonal antibody to Cytochrome P450 1A1/2 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Cytochrome P450 1A1/2. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200) Gene Symbol: CYP1A1; CYP1A2 Alternative Names: CYP1A1; Cytochrome P450 1A1; CYPIA1; Cytochrome P450 form 6; Cytochrome P450-C; Cytochrome P450-P1; CYP1A2; Cytochrome P450 1A2; CYPIA2; Cytochrome P(3)450; Cytochrome P450 4; Cytochrome P450-P3Entrez Gene (Human): 1543; 1544Entrez Gene (Mouse) : 13076; 13077Entrez Gene (Rat) : 24296; 24297SwissProt (Human): P04798; P05177SwissProt (Mouse) : P00184; P00186SwissProt (Rat) : P00185; P04799Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Cytochrome P450 1A1/2 expression in MCF7 (A), HeLa (B) whole cell lysates.
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  Immunofluorescent analysis of Cytochrome P450 1A1/2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).