Anti-CBP Antibody-Primary Antibody-Cohesion Biosciences

Western blot analysis of CBP expression in HeLa (A), A431 (B), HT29 (C), A549 (D) whole cell lysates.
Immunohistochemical analysis of CBP staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of CBP staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Product Name	Anti-CBP Antibody
Cat No:	CPA1273
Source:	Rabbit
Reactivity:	H, M, R, B, C, D, P
Applications:	WB, IH, IF/IC
*Application Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
*Species Reactivity Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish

Size

Price

200 μl

$350

100 μl

$220

30 μl

$110

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Description: Rabbit polyclonal antibody to CBP

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human CBP. The exact sequence is proprietary.

Purification: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)

Gene Symbol: CREBBP

Alternative Names: CBP; CREB-binding protein

Entrez Gene (Human): 1387

SwissProt (Human): Q92793

SwissProt (Mouse) : P45481

SwissProt (Rat) : Q6JHU9

Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Western blot analysis of CBP expression in HeLa (A), A431 (B), HT29 (C), A549 (D) whole cell lysates.
Immunohistochemical analysis of CBP staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of CBP staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Progesterone Receptor Coregulators as Factors Supporting the Function of the Corpus Luteum in Cows

Journal Genes

IF 3.759

Application WB

Reactivity Bovine

PMID 32806523

Steroid Receptor Coregulators Can Modulate the Action of Progesterone Receptor during the Estrous Cycle in Cow Endometrium

Journal Animals

IF 2.752

Application WB, IHC

Reactivity Bovine

PMID 34827948

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