Description: Rabbit polyclonal antibody to CNPase Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CNPase. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: CNP Alternative Names: 2',3'-cyclic-nucleotide 3'-phosphodiesterase; CNP; CNPaseEntrez Gene (Human): 1267Entrez Gene (Mouse) : 12799Entrez Gene (Rat) : 25275SwissProt (Human): P09543SwissProt (Mouse) : P16330SwissProt (Rat) : P13233Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CNPase expression in mouse brain (A), PC12 (B), CT12 (C), SGC7901 (D), K562 (E) whole cell lysates.
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Immunohistochemical analysis of CNPase staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.