Description: Rabbit polyclonal antibody to IKK alpha (Phospho-T23) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T23 of human IKK alpha protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: CHUK Alternative Names: IKKA; TCF16; Inhibitor of nuclear factor kappa-B kinase subunit alpha; I-kappa-B kinase alpha; IKK-A; IKK-alpha; IkBKA; IkappaB kinase; Conserved helix-loop-helix ubiquitous kinase; I-kappa-B kinase 1; IKK1; Nuclear factor NF-kappa-B inhibitor kinase alpha; NFKBIKA; Transcription factor 16; TCF-16Entrez Gene (Human): 1147Entrez Gene (Mouse) : 12675SwissProt (Human): O15111SwissProt (Mouse) : Q60680Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

• 
  Western blot analysis of IKK alpha (Phospho-T23) expression in HeLa TNFa-treated (A), Raw264.7 TNFa-treated (B), PC12 LPS-treated (C) whole cell lysates.
• 
  Immunohistochemical analysis of IKK alpha (Phospho-T23) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.