Description: Rabbit polyclonal antibody to CDC20 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CDC20. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: CDC20 Alternative Names: Cell division cycle protein 20 homolog; p55CDCEntrez Gene (Human): 991SwissProt (Human): Q12834Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CDC20 expression in Hela (A), DLD (B), LOVO (C), mouse spleen (D), rat spleen (E) whole cell lysates.
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Immunohistochemical analysis of CDC20 staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
APC15 mediates CDC20 autoubiquitylation by APC/C(MCC) and disassembly of the mitotic checkpoint complex
Persistent telomere damage induces bypass of mitosis and tetraploidy