Anti-Cyclin B1 (Phospho-S126) Antibody 
Datasheet
MSDS
Description: Rabbit polyclonal antibody to Cyclin B1 (Phospho-S126) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S126 of human Cyclin B1 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: CCNB1 Alternative Names: CCNB; G2/mitotic-specific cyclin-B1Entrez Gene (Human): 891Entrez Gene (Mouse) : 268697SwissProt (Human): P14635SwissProt (Mouse) : P24860Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Cyclin B1 (Phospho-S126) expression in K562 (A) whole cell lysates. -
Immunohistochemical analysis of Cyclin B1 (Phospho-S126) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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