Description: Rabbit polyclonal antibody to c-CBL (Phospho-Y674) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y674 of human c-CBL protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: CBL Alternative Names: CBL2; RNF55; E3 ubiquitin-protein ligase CBL; Casitas B-lineage lymphoma proto-oncogene; Proto-oncogene c-Cbl; RING finger protein 55; Signal transduction protein CBLEntrez Gene (Human): 867Entrez Gene (Mouse) : 12402SwissProt (Human): P22681SwissProt (Mouse) : P22682Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of c-CBL (Phospho-Y674) expression in HeLa EGF-treated (A), mouse liver (B), rat liver (C) whole cell lysates.
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  Immunohistochemical analysis of c-CBL (Phospho-Y674) staining in human cervical cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.