Description: Rabbit polyclonal antibody to Caveolin 1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Caveolin 1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500) Gene Symbol: CAV1 Alternative Names: CAV; Caveolin-1Entrez Gene (Human): 857Entrez Gene (Mouse) : 12389SwissProt (Human): Q03135SwissProt (Mouse) : P49817SwissProt (Rat) : P41350Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Caveolin 1 expression in Hela (A), H1688 (B), mouse lung (C), rat lung (D) whole cell lysates.
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Immunofluorescent analysis of Caveolin 1 staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).