Description: Rabbit polyclonal antibody to Calcium Sensing Receptor (Phospho-T888) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T888 of human Calcium Sensing Receptor protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200) Gene Symbol: CASR Alternative Names: GPRC2A; PCAR1; Extracellular calcium-sensing receptor; CaSR; Parathyroid cell calcium-sensing receptor 1; PCaR1Entrez Gene (Human): 846Entrez Gene (Mouse) : 12374Entrez Gene (Rat) : 24247SwissProt (Human): P41180SwissProt (Mouse) : Q9QY96SwissProt (Rat) : P48442Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of Calcium Sensing Receptor (Phospho-T888) expression in MCF7 (A), MDCK (B), U2OS (C), SP2/0 (D), PC12 (E), rat brain (F) whole cell lysates.
-
Immunofluorescent analysis of Calcium Sensing Receptor (Phospho-T888) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
A calcium-induced signaling cascade leading to osteogenic differentiation of human bone marrow-derived mesenchymal stromal cells