Description: Rabbit polyclonal antibody to Caspase 4 p20 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Caspase 4 p20. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: CASP4 Alternative Names: ICH2; Caspase-4; CASP-4; ICE(rel)-II; Protease ICH-2; Protease TXEntrez Gene (Human): 837Entrez Gene (Mouse) : 12363SwissProt (Human): P49662SwissProt (Mouse) : P70343Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Caspase 4 p20 expression in HepG2 colchicine-treated (A), SP2/0 H2O2-treated (B), rat kidney (C) whole cell lysates.
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  Immunohistochemical analysis of Caspase 4 p20 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.