Description: Rabbit polyclonal antibody to active Caspase 3 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Caspase 3. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: CASP3 Alternative Names: CPP32; Caspase-3; CASP-3; Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1; SCA-1Entrez Gene (Human): 836Entrez Gene (Mouse) : 12367Entrez Gene (Rat) : 25402SwissProt (Human): P42574SwissProt (Mouse) : P70677SwissProt (Rat) : P55213Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of active Caspase 3 expression in HepG2 colchicine-treated (A), mouse liver (B), rat liver (C), Jurkat etoposide-treated (D), NIH3T3 staurosporine-treated (E), HeLa (F) whole cell lysates. (Predicted band size: 31 kD; Observed band size: 17 kD)
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  Immunohistochemical analysis of active Caspase 3 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.