Description: Rabbit polyclonal antibody to Carbonic Anhydrase 1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Carbonic Anhydrase 1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: CA1 Alternative Names: Carbonic anhydrase 1; Carbonate dehydratase I; Carbonic anhydrase B; CAB; Carbonic anhydrase I; CA-IEntrez Gene (Human): 759Entrez Gene (Mouse) : 12346Entrez Gene (Rat) : 310218SwissProt (Human): P00915SwissProt (Mouse) : P13634SwissProt (Rat) : B0BNN3Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Carbonic Anhydrase 1 expression in rat spleen (A) whole cell lysates.
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Immunohistochemical analysis of Carbonic Anhydrase 1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.