Description: Rabbit polyclonal antibody to BRCA1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human BRCA1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: BRCA1 Alternative Names: RNF53; Breast cancer type 1 susceptibility protein; RING finger protein 53Entrez Gene (Human): 672Entrez Gene (Mouse) : 12189Entrez Gene (Rat) : 497672SwissProt (Human): P38398SwissProt (Mouse) : P48754SwissProt (Rat) : O54952Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of BRCA1 expression in HeLa (A), mouse kidney (B), rat brain (C) whole cell lysates.
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  Immunohistochemical analysis of BRCA1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.