Description: Rabbit polyclonal antibody to BNIP2 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human BNIP2. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: BNIP2 Alternative Names: NIP2; BCL2/adenovirus E1B 19 kDa protein-interacting protein 2Entrez Gene (Human): 663Entrez Gene (Mouse) : 12175SwissProt (Human): Q12982SwissProt (Mouse) : O54940Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of BNIP2 expression in A549 (A), HUT78 (B), mouse kidney (C) whole cell lysates.
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Immunohistochemical analysis of BNIP2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.