Description: Rabbit polyclonal antibody to ATP6V1B1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human ATP6V1B1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: ATP6V1B1 Alternative Names: ATP6B1; VATB; VPP3; V-type proton ATPase subunit B, kidney isoform; V-ATPase subunit B 1; Endomembrane proton pump 58 kDa subunit; Vacuolar proton pump subunit B 1Entrez Gene (Human): 525SwissProt (Human): P15313Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of ATP6V1B1 expression in mouse lung (A), mouse kidney (B), rat lung (C), rat kidney (D) whole cell lysates.
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Immunohistochemical analysis of ATP6V1B1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.