Description: Rabbit polyclonal antibody to ATP5A1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ATP5A1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: ATP5A1 Alternative Names: ATP5A; ATP5AL2; ATPM; ATP synthase subunit alpha mitochondrialEntrez Gene (Human): 498Entrez Gene (Mouse) : 11946Entrez Gene (Rat) : 65262SwissProt (Human): P25705SwissProt (Mouse) : Q03265SwissProt (Rat) : P15999Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of ATP5A1 expression in HEK293T (A), Hela (B), H446 (C), mouse lung (D), mouse liver (E), rat lung (F), rat liver (G) whole cell lysates.
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  Immunohistochemical analysis of ATP5A1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.