Description: Rabbit polyclonal antibody to APE1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human APE1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: APEX1 Alternative Names: APE; APE1; APEX; APX; HAP1; REF1; DNA-(apurinic or apyrimidinic site) lyase; APEX nuclease; APEN; Apurinic-apyrimidinic endonuclease 1; AP endonuclease 1; APE-1; REF-1; Redox factor-1Entrez Gene (Human): 328Entrez Gene (Mouse) : 11792Entrez Gene (Rat) : 79116SwissProt (Human): P27695SwissProt (Mouse) : P28352SwissProt (Rat) : P43138Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of APE1 expression in Hela (A), A549 (B), rat lung (C) whole cell lysates.
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  Immunohistochemical analysis of APE1 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.