Description: Rabbit polyclonal antibody to AKT (Phospho-T308) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T308 of human AKT protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: AKT1; AKT2; AKT3 Alternative Names: AKT1; PKB; RAC; RAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha; AKT2; RAC-beta serine/threonine-protein kinase; Protein kinase Akt-2; Protein kinase B beta; PKB beta; RAC-PK-beta; AKT3; PKBG; RAC-gamma serine/threonine-protein kinase; Protein kinase Akt-3; Protein kinase B gamma; PKB gamma; RAC-PK-gamma; STK-2Entrez Gene (Human): 207; 208; 10000Entrez Gene (Mouse) : 11651; 11652; 23797Entrez Gene (Rat) : 24185; 25233; 29414SwissProt (Human): P31749; P31751; Q9Y243SwissProt (Mouse) : P31750; Q60823; Q9WUA6SwissProt (Rat) : P47196; P47197; Q63484Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of AKT (Phospho-T308) expression in HeLa colchicine-treated (A), HL60 (B), NIH3T3 (C), SP2/0 colchicine-treated (D), PC12 colchicine-treated (E) whole cell lysates.
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  Immunohistochemical analysis of AKT (Phospho-T308) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Direct ELISA antibody dose-response curve using Anti-AKT (Phospho-T308) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.