Description: Rabbit polyclonal antibody to ACTN3 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human ACTN3. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500) Gene Symbol: ACTN3 Alternative Names: Alpha-actinin-3; Alpha-actinin skeletal muscle isoform 3; F-actin cross-linking proteinEntrez Gene (Human): 89Entrez Gene (Mouse) : 11474SwissProt (Human): Q08043SwissProt (Mouse) : O88990Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

• 
  Western blot analysis of ACTN3 expression in HeLa (A), mouse muscle (B), rat muscle (C) whole cell lysates.
• 
  Immunofluorescent analysis of ACTN3 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).