Description: Rabbit polyclonal antibody to Gamma-actin-1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Gamma-actin-1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200) Gene Symbol: ACTG1 Alternative Names: ACTB; Actin, cytoplasmic 1; Beta-actin; POTEKP; ACTBL3; FKSG30; Putative beta-actin-like protein 3; Kappa-actin; POTE ankyrin domain family member K; ACTG1; ACTB; ACTG; Actin, cytoplasmic 2; Gamma-actinEntrez Gene (Human): 60; 71Entrez Gene (Mouse) : 11461; 11465Entrez Gene (Rat) : 81822; 100361457SwissProt (Human): P60709; Q9BYX7; P63261SwissProt (Mouse) : P60710; P63260SwissProt (Rat) : P60711; P63259Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Gamma-actin-1 expression in HEK293T (A), Hela (B), mouse brain (C), rat muscle (D), rat brain (E), rat spleen (F) whole cell lysates.
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  Immunohistochemical analysis of Gamma-actin-1 staining in human muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.