Description: Recombinant rabbit monoclonal antibody to CD14 Immunogen: Recombinant fusion protein of human CD14. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/500) Gene Symbol: CD14 Alternative Names: Monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein; CD14Entrez Gene (Human): 929Entrez Gene (Mouse) : 12475Entrez Gene (Rat) : 60350SwissProt (Human): P08571SwissProt (Mouse) : P10810SwissProt (Rat) : Q63691Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of CD14 expression in HepG2 (A), THP1 (B), MCF7 (C), mouse liver (D), rat kidney (E), rat liver (F) whole cell lysates. (Predicted band size: 40 kD; Observed band size: 50-55, 40 kD)
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  Immunohistochemical analysis of CD14 staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.