Description: Rabbit polyclonal antibody to EDG4 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human EDG4. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200) Gene Symbol: LPAR2 Alternative Names: EDG4; LPA2; Lysophosphatidic acid receptor 2; LPA receptor 2; LPA-2; Lysophosphatidic acid receptor Edg-4Entrez Gene (Human): 9170SwissProt (Human): Q9HBW0SwissProt (Mouse) : Q9JL06Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of EDG4 expression in HEK293T (A), PC3 (B), Panc1 (C), mouse liver (D), mouse muscle (E), rat liver (F), rat muscle (G) whole cell lysates. (Predicted band size: 38 kD; Observed band size: 45 kD)
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  Immunofluorescent analysis of EDG4 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.