Description: Rabbit polyclonal antibody to MRCK beta Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human MRCK beta. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: CDC42BPB Alternative Names: KIAA1124; Serine/threonine-protein kinase MRCK beta; CDC42-binding protein kinase beta; CDC42BP-beta; DMPK-like beta; Myotonic dystrophy kinase-related CDC42-binding kinase beta; MRCK beta; Myotonic dystrophy protein kinase-like betaEntrez Gene (Human): 9578Entrez Gene (Mouse) : 217866Entrez Gene (Rat) : 113960SwissProt (Human): Q9Y5S2SwissProt (Mouse) : Q7TT50SwissProt (Rat) : Q7TT49Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of MRCK beta expression in HEK293T (A), mouse muscle (B), rat muscle (C) whole cell lysates. (Predicted band size: 194 kD; Observed band size: 230 kD)
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Immunohistochemical analysis of MRCK beta staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.