Description: Rabbit polyclonal antibody to NF-kappaB p65 (DiMethyl-K221) Immunogen: KLH-conjugated synthetic acetylated peptide corresponding to residues surrounding K221 of human NF-kappaB p65. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000) Gene Symbol: RELA Alternative Names: NFKB3; Transcription factor p65; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3Entrez Gene (Human): 5970SwissProt (Human): Q04206Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of NF-kappaB p65 (DiMethyl-K221) expression in EC9706 (A), Myla2059 (B) whole cell lysates. (Predicted band size: 60 kD; Observed band size: 75 kD)
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  Direct ELISA antibody dose-response curve using Anti-NF-kappaB p65 (DiMethyl-K221) Antibody. Antigen (methyl-peptide and non-methyl-peptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.