Description: Rabbit polyclonal antibody to Heparanase Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Heparanase. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: HPSE Alternative Names: HEP; HPA; HPA1; HPR1; HPSE1; HSE1; Heparanase; Endo-glucoronidase; Heparanase-1; Hpa1Entrez Gene (Human): 10855Entrez Gene (Mouse) : 15442Entrez Gene (Rat) : 64537SwissProt (Human): Q9Y251SwissProt (Mouse) : Q6YGZ1SwissProt (Rat) : Q71RP1Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Heparanase expression in mouse liver (A), rat liver (B) whole cell lysates. (Predicted band size: 61, 53, 42 kD; Observed band size: 61, 42 kD)
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Immunohistochemical analysis of Heparanase staining in human pancreas formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.