Description: Recombinant rabbit monoclonal antibody to p34-ARC Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human p34-ARC. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: ARPC2 Alternative Names: ARC34; Actin-related protein 2/3 complex subunit 2; Arp2/3 complex 34 kDa subunit; p34-ARCEntrez Gene (Human): 10109Entrez Gene (Mouse) : 76709SwissProt (Human): O15144SwissProt (Mouse) : Q9CVB6SwissProt (Rat) : P85970Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of p34-ARC expression in THP1 (A), A549 (B), Hela (C), mouse liver (D), mouse muscle (E), rat kidney (F), rat muscle (G) whole cell lysates. (Predicted band size: 34 kD; Observed band size: 34 kD)
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Immunohistochemical analysis of p34-ARC staining in human squamous cell carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.