Description: Recombinant rabbit monoclonal antibody to UQCRH Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human UQCRH. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200) Gene Symbol: UQCRH Alternative Names: Cytochrome b-c1 complex subunit 6, mitochondrial; Complex III subunit 6; Complex III subunit VIII; Cytochrome c1 non-heme 11 kDa protein; Mitochondrial hinge protein; Ubiquinol-cytochrome c reductase complex 11 kDa proteinEntrez Gene (Human): 7388Entrez Gene (Rat) : 366448SwissProt (Human): P07919SwissProt (Rat) : Q5M9I5Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of UQCRH expression in rat kidney (A) whole cell lysates. (Predicted band size: 10 kD; Observed band size: 11 kD)
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  Immunofluorescent analysis of UQCRH staining in COS7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).