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Recombinant Anti-SEC14L2 Rabbit mAb CMA4441
  • Western blot analysis of SEC14L2 expression in PC3 (A), Hela (B), HepG2 (C), mouse liver (D), mouse muscle (E), rat liver (F), rat muscle (G) whole cell lysates. (Predicted band size: 46 kD; Observed band size: 46 kD)
  • Immunofluorescent analysis of SEC14L2 staining in A375 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
Product NameRecombinant Anti-SEC14L2 Rabbit mAb
Cat No: CMA4441
Source: Rabbit
Reactivity: H, M, R
Applications: WB, IF/IC
*Application Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
*Species Reactivity Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
Size
Price
200 μl
$350
100 μl
$220
30 μl
$110
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Description: Recombinant rabbit monoclonal antibody to SEC14L2
Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human SEC14L2. The exact sequence is proprietary.
Purification: The antibody was purified by immunogen affinity chromatography.
Clonality: Monoclonal
Form: Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.
Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)
Gene Symbol: SEC14L2
Alternative Names: C22orf6; KIAA1186; KIAA1658; SEC14-like protein 2; Alpha-tocopherol-associated protein; TAP; hTAP; Squalene transfer protein; Supernatant protein factor; SPF
Entrez Gene (Human): 23541
Entrez Gene (Mouse) : 67815
SwissProt (Human): O76054
SwissProt (Mouse) : Q99J08
SwissProt (Rat) : Q99MS0
Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of SEC14L2 expression in PC3 (A), Hela (B), HepG2 (C), mouse liver (D), mouse muscle (E), rat liver (F), rat muscle (G) whole cell lysates. (Predicted band size: 46 kD; Observed band size: 46 kD)
  • Immunofluorescent analysis of SEC14L2 staining in A375 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
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