Description: Recombinant rabbit monoclonal antibody to CRCP Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human CRCP. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200) Gene Symbol: CRCP Alternative Names: DNA-directed RNA polymerase III subunit RPC9; RNA polymerase III subunit C9; Calcitonin gene-related peptide-receptor component protein; CGRP-RCP; CGRP-receptor component protein; CGRPRCP; HsC17Entrez Gene (Human): 27297Entrez Gene (Mouse) : 12909SwissProt (Human): O75575SwissProt (Mouse) : O35427Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of CRCP expression in MCF7 (A), THP1 (B), mouse liver (C), mouse kidney (D) whole cell lysates. (Predicted band size: 16 kD; Observed band size: 22 kD)
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  Immunofluorescent analysis of CRCP staining in A375 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).