Description: Recombinant rabbit monoclonal antibody to Histone H2B (Formyl-K108) Immunogen: KLH-conjugated synthetic formylated peptide corresponding to residues surrounding K108 of human Histone H2B protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: HIST2H2BE Alternative Names: H2BFQ; Histone H2B type 2-E; Histone H2B-GL105; Histone H2B.q; H2B/qEntrez Gene (Human): 8349Entrez Gene (Mouse) : 319190SwissProt (Human): Q16778SwissProt (Mouse) : Q64524Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Histone H2B (Formyl-K108) expression in K562 (A), MCF7 (B), Jurkat (C), mouse liver (D), mouse kidney (E), rat liver (F), rat kidney (G) whole cell lysates. (Predicted band size: 13 kD; Observed band size: 14 kD)
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Immunohistochemical analysis of Histone H2B (Formyl-K108) staining in human Brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.