Description: Recombinant rabbit monoclonal antibody to MAD2L2 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human MAD2L2. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200) Gene Symbol: MAD2L2 Alternative Names: MAD2B; REV7; Mitotic spindle assembly checkpoint protein MAD2B; Mitotic arrest deficient 2-like protein 2; MAD2-like protein 2; REV7 homolog; hREV7Entrez Gene (Human): 10459Entrez Gene (Mouse) : 71890Entrez Gene (Rat) : 313702SwissProt (Human): Q9UI95SwissProt (Mouse) : Q9D752SwissProt (Rat) : D3Z8D9Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of MAD2L2 expression in K562 (A), Jurkat (B), THP1 (C), mouse kidney (D), muuse muscle (E), rat kidney (F), rat muscle (G) whole cell lysates. (Predicted band size: 24 kD; Observed band size: 24 kD)
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  Immunofluorescent analysis of MAD2L2 staining in A375 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).