Description: Recombinant rabbit monoclonal antibody to Hemoglobin gamma-1/2 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human Hemoglobin gamma-1/2. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200) Gene Symbol: HBG1; HBG2 Alternative Names: Hemoglobin subunit gamma-1; Gamma-1-globin; Hb F Agamma; Hemoglobin gamma-1 chain; Hemoglobin gamma-A chain; Hemoglobin subunit gamma-2; Gamma-2-globin; Hb F Ggamma; Hemoglobin gamma-2 chain; Hemoglobin gamma-G chainEntrez Gene (Human): 3047; 3048SwissProt (Human): P69891; P69892Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Hemoglobin gamma-1/2 expression in K562 (A) whole cell lysates. (Predicted band size: 16 kD; Observed band size: 12 kD)
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  Immunofluorescent analysis of Hemoglobin gamma-1/2 staining in COS7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).