Description: Recombinant rabbit monoclonal antibody to TOE1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human TOE1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: TOE1 Alternative Names: Target of EGR1 protein 1Entrez Gene (Human): 114034SwissProt (Human): Q96GM8Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of TOE1 expression in Hela (A), THP1 (B) whole cell lysates. (Predicted band size: 56 kD; Observed band size: 60 kD)
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  Immunohistochemical analysis of TOE1 staining in human squamous cell carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. Tyramide-AF488 (green) was used as the chromogen. The section was then counterstained with DAPI (blue).