Description: Recombinant rabbit monoclonal antibody to E2F1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human E2F1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200) Gene Symbol: E2F1 Alternative Names: RBBP3; Transcription factor E2F1; E2F-1; PBR3; Retinoblastoma-associated protein 1; RBAP-1; Retinoblastoma-binding protein 3; RBBP-3; pRB-binding protein E2F-1Entrez Gene (Human): 1869Entrez Gene (Mouse) : 13555SwissProt (Human): Q01094SwissProt (Mouse) : Q61501SwissProt (Rat) : O09139Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of E2F1 expression in Hela (A), Jurkat (B), THP1 (C), mouse liver (D), mouse muscle (E), rat liver (F) whole cell lysates. (Predicted band size: 46 kD; Observed band size: 65 kD)
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  Immunofluorescent analysis of E2F1 staining in A375 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).