Description: Recombinant rabbit monoclonal antibody to PAX6 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human PAX6. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200) Gene Symbol: PAX6 Alternative Names: AN2; Paired box protein Pax-6; Aniridia type II protein; OculorhombinEntrez Gene (Human): 5080Entrez Gene (Mouse) : 18508Entrez Gene (Rat) : 25509SwissProt (Human): P26367SwissProt (Mouse) : P63015SwissProt (Rat) : P63016Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of PAX6 expression in Hela (A), Jurkat (B), K562 (C), mouse brain (D), rat brain (E), rat muscle (F) whole cell lysates. (Predicted band size: 46 kD; Observed band size: 46; 48KD)
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  Immunofluorescent analysis of PAX6 staining in A375 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with an AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).