Description: Recombinant rabbit monoclonal antibody to STAG2 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human STAG2. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: STAG2 Alternative Names: SA2; Cohesin subunit SA-2; SCC3 homolog 2; Stromal antigen 2Entrez Gene (Human): 10735SwissProt (Human): Q8N3U4Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of STAG2 expression in HEK293T (A), K562 (B) whole cell lysates. (Predicted band size: 141 kD; Observed band size: 141 kD)
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Immunohistochemical analysis of STAG2 staining in human kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. Tyramide-AF488 (green) was used as the chromogen. The section was then counterstained with DAPI (blue).