Description: Recombinant rabbit monoclonal antibody to SMAD2 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human SMAD2. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: SMAD2 Alternative Names: MADH2; MADR2; Mothers against decapentaplegic homolog 2; MAD homolog 2; Mothers against DPP homolog 2; JV18-1; Mad-related protein 2; hMAD-2; SMAD family member 2; SMAD 2; Smad2; hSMAD2Entrez Gene (Human): 4087Entrez Gene (Mouse) : 17126SwissProt (Human): Q15796SwissProt (Mouse) : Q62432Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of SMAD2 expression in HEK293T (A), THP1 (B), mouse liver (C) whole cell lysates. (Predicted band size: 52 kD; Observed band size: 58 kD)
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  Immunohistochemical analysis of SMAD2 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. Tyramide-AF488 (green) was used as the chromogen. The section was then counterstained with DAPI (blue).