Description: Recombinant rabbit monoclonal antibody to ACAT1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human ACAT1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: ACAT1 Alternative Names: ACAT; MAT; Acetyl-CoA acetyltransferase, mitochondrial; Acetoacetyl-CoA thiolase; T2Entrez Gene (Human): 38Entrez Gene (Mouse) : 110446Entrez Gene (Rat) : 25014SwissProt (Human): P24752SwissProt (Mouse) : Q8QZT1SwissProt (Rat) : P17764Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of ACAT1 expression in HEK293T (A), PC3 (B), THP1 (C), mouse kidney (D), rat liver (E), rat kidney (F) whole cell lysates. (Predicted band size: 45 kD; Observed band size: 45 kD)
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  Immunohistochemical analysis of ACAT1 staining in human stomach cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. Tyramide-AF488 (green) was used as the chromogen. The section was then counterstained with DAPI (blue).