Description: Recombinant rabbit monoclonal antibody to RhoA/B/C Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human RhoA/B/C. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: RHOA; RHOB; RHOC Alternative Names: ARH12; ARHA; RHO12; Transforming protein RhoA; Rho cDNA clone 12; h12; ARH6; ARHB; Rho-related GTP-binding protein RhoB; Rho cDNA clone 6; h6; ARH9; ARHC; Rho-related GTP-binding protein RhoC; Rho cDNA clone 9; h9Entrez Gene (Human): 387; 388; 389Entrez Gene (Mouse) : 11848; 11852; 11853Entrez Gene (Rat) : 117273SwissProt (Human): P61586; P62745; P08134SwissProt (Mouse) : Q9QUI0; P62746; Q62159SwissProt (Rat) : P61589Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of RhoA/B/C expression in HEK293T (A), mouse kidney (B), rat kidney (C) whole cell lysates. (Predicted band size: 21 kD; Observed band size: 22 kD)
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  Immunohistochemical analysis of RhoA/B/C staining in rat kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. Tyramide-AF488 (green) was used as the chromogen. The section was then counterstained with DAPI (blue).