Description: Recombinant rabbit monoclonal antibody to ERK2 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human ERK2. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: MAPK1 Alternative Names: ERK2; PRKM1; PRKM2; Mitogen-activated protein kinase 1; MAP kinase 1; MAPK 1; ERT1; Extracellular signal-regulated kinase 2; ERK-2; MAP kinase isoform p42; p42-MAPK; Mitogen-activated protein kinase 2; MAP kinase 2; MAPK 2Entrez Gene (Human): 5594Entrez Gene (Mouse) : 26413Entrez Gene (Rat) : 116590SwissProt (Human): P28482SwissProt (Mouse) : P63085SwissProt (Rat) : P63086Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of ERK2 expression in HEK293T (A), K562 (B), THP1 (C), mouse kidney (D), mouse muscle (E), rat kidney (F), rat muscle (G) whole cell lysates. (Predicted band size: 41 kD; Observed band size: 41 kD)
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  Immunohistochemical analysis of ERK2 staining in rat kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. Tyramide-AF488 (green) was used as the chromogen. The section was then counterstained with DAPI (blue).