Description: Recombinant rabbit monoclonal antibody to UBA52 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human UBA52. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Monoclonal Form: Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: UBA52 Alternative Names: UBCEP2; Ubiquitin-60S ribosomal protein L40; CEP52; Ubiquitin A-52 residue ribosomal protein fusion product 1Entrez Gene (Human): 7311Entrez Gene (Mouse) : 22186SwissProt (Human): P62987SwissProt (Mouse) : P62984Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of UBA52 expression in HEK293T (A), K562 (B), mouse liver (C), mouse muscle (D) whole cell lysates. (Predicted band size: 14 kD; Observed band size: 10 kD)
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  Immunohistochemical analysis of UBA52 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.